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PLATE 39.

Bacillus subtilis (Ehrenberg). F. Cohn.

(Hay Bacillus.)

I. Gelatin stab culture, thirty-six hours at 22°.
II. Gelatin stab culture, eight days at 22°.
III. Agar streak culture, two days at 37°.
IV. Agar stab culture, two days at 37°.
V. Agar stab culture, two days at 37°.
VI. Agar plate, twelve hours at 37°.
ficial colony.

VII. Agar plate, twelve hours at 37°. colony.

Stab canal.
Surface growth.

X 60. Super

X 60. Deep

VIII. Agar plate, twelve hours at 37°. Natural size.

PLATE 40.

Bacillus subtilis (Ehrenberg). F. Cohn.

(Hay Bacillus.)

I. Potato culture, seven days at 22°.

II. Gelatin plate, two days at 22°. × 60. Above and to the left, a deep colony; below this, one lying directly at the surface; to the right, a superficial colony.

III. Gelatin plate, two days at 22°. Natural size.

IV. Gelatin plate, two days at 22°. X 10.

V. Microscopic preparation. × 1000. From an agar culture three hours old at 37°, stained with fuchsin.

VI. Microscopic preparation. Bacilli with flagella, after Fischer. Very highly magnified.

From an agar Unstained.

From an agar

VII. Microscopic preparation. × 1000. culture, ten days at 22°. Contains spores. VIII. Microscopic preparation. X 700. culture, ten days at 22°. Double stain with carbol-fuchsin and methylene-blue.

IX. Bacilli with numerous flagella. 1000. Stained according to Löffler.

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