Bacillus tetani.

PLATE 44.

Nicolaier. (Tetanus Bacillus.)

I. Sugar-agar stab culture, three days at 37°. II. Sugar-gelatin stab culture, six days at 22°. III. Sugar-gelatin plate, four days at 22°. without air.

Grown

IV. Sugar-gelatin plate, four days at 22°. × 60. Natural size. Superficial and deep colonies. Grown without air. V. Sugar-agar plate, four days at 37°. Natural size. Grown without air.

VI. Sugar-agar plate, four days at 37°. X 60. Superficial and deep colonies. Grown without air.

VII. Microscopic preparation. Pure culture on sugaragar, three days at 37°. × 1000. Bacilli with spores. Double staining according to Ziehl.

VIII. Microscopic preparation. Pure culture on sugaragar, two days at 37°. X 1000. Some bacilli contain spores. Stained with fuchsin.

IX. Microscopic preparation. Pure culture on sugaragar, twenty-four hours at 37°. × 1000. Extremely long

threads with faintly stained intervals.

X. Microscopic preparation. Pure culture on sugaragar, six days at 37°. X 1000. Stained with fuchsin. Long threads and chains of spores with faintly stained inter

vals.

XI. Microscopic Highly magnified.

preparation.

Flagella staining.