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Sarcina flava. De Bary, emended by Lehm. and Stu

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I. Gelatin stab culture, ten days at 22o.
II. Agar streak culture, six days at 22o.
III. Agar stab culture, six days at 22°; stab canal.
IV. Agar stab culture, six days at 22°; surface growth.

V. Gelatin plate, five days at 22o. Natural size.

VI. Gelatin plate, five days at 22o. X 60. Superficial colony.

VII. Agar plate, six days at 22o. Natural size. VIII. Agar plate, six days at 22o. X 60. Upper colony is superficial, lower ones are deep.

IX. Potato culture, ten days at 22o.

X. Microscopic preparation. Pure culture from an agar plate. X 1000. Stained with fuchsin and differentiated with acetic acid.

XI. Microscopic preparation. Pure culture in bouillon; unstained. X 1000.

XII. Sarcinæ forming bales of packets. (Single packets regularly grouped together.)

XIII. Sarcina in bunches of packets. (Single or irregular packets, grouped together irregularly.)

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Sarcina aurantiaca. Flügge.
I. Gelatin stab culture, six days at 22o.

II. Agar streak culture, five days at 22o. The color is not so red in all cases; usually light orange. This is also true of the agar stab and potato cultures.

III. Agar stab culture, six days at 22°. Stab canal.

IV. Agar stab culture, six days at 22o. Surface growth.

V. Gelatin plate, five days at 22o. Natural size. The gray zone about the colonies indicates a depression. VI. Gelatin plate, five days at 22° X 60. A colony

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A in the early stage. The gray ring represents a zone where it is sinking in.

VII. Agar plate, five days at 22o. Natural size.
VIII. Agar plate, five days at 22°. X 60. Upper

, . X colony, superficial; lower colonies, deep. The superficial colonies usually become opaque toward the center.

IX. Potato culture, eight days old.

X. Microscopic preparation. Pure culture from agar. X 1000. Stained with fuchsin, differentiated with acetic acid.

XI. Microscopic preparation. Pure culture in bouillon. X 1000. Unstained, partly schematic.

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