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Micrococcus gonorrhææ. Neisser. Bumm.
I. Agar streak culture, ascites-glycerin-agar, three days at 37o.
II. Agar plate, forty-eight hours at 37o. X 60. Superficial colonies. The agar was poured out, and blood from the finger-tip smeared upon it, and upon this was placed the gonorrheal pus. The reddish places are blood. The colonies of the gonococcus grow principally at the periphery of the blood smear.
III. Serum-agar plate. The upper colony three days, the lower twenty-four hours, at 37° X 60. Superficial colonies. One c.c. of human serum was added to the agar.
IV. Serum-agar plate. The same colonies after eight days.
V. Ascites-glycerin-agar plate, forty-eight hours at 37o. X 60. Superficial colonies of a pure culture from blennorrheal pus. To 5 c.c. of a 2% agar, containing 5% of glycerin, 1.5 c.c. of human ascites-fluid were added.
VI. Ascites-glycerin-agar plate, forty-eight hours, at 37°. X 60. Superficial colonies. After pouring out the agar, blennorrheal pus was smeared upon it. The darker septa are pus (pushed together by the growing colonies); also the material at the periphery of the colonies.
VII. Smear preparation from gonorrheal pus. X 1000. . Stained with methylene-blue.
VIII. Smear preparation from blennorrheal pus. X 1000. Stained with methylene-blue. A pus cell in which lie the micrococci, almost always in fours in capsules. The preparation contains a great many micrococci thus situated.
IX. Smear preparation from blennorrheal pus. X 1000. Stained with methylene-blue and eosin.
X. Micrococci, highly magnified, schematic.