PLATE 3. Sarcina flava. De Bary, emended by Lehm. and Stubenrath. I. Gelatin stab culture, ten days at 22°. III. Agar stab culture, six days at 22°; stab canal. VII. Agar plate, six days at 22°. Natural size. VIII. Agar plate, six days at 22°. 60. Upper colony is superficial, lower ones are deep. IX. Potato culture, ten days at 22°. X. Microscopic preparation. Pure culture from an agar plate. X 1000. Stained with fuchsin and differentiated with acetic acid. XI. Microscopic preparation. Pure culture in bouillon; unstained. X 1000. XII. Sarcina forming bales of packets. (Single packets regularly grouped together.) XIII. Sarcina in bunches of packets. (Single or irregular packets, grouped together irregularly.) PLATE 4. Sarcina aurantiaca. Flügge. I. Gelatin stab culture, six days at 22°. II. Agar streak culture, five days at 22°. not so red in all cases; usually light orange. true of the agar stab and potato cultures. The color is III. Agar stab culture, six days at 22°. Stab canal. IV. Agar stab culture, six days at 22°. Surface growth. V. Gelatin plate, five days at 22°. Natural size. The gray zone about the colonies indicates a depression. VI. Gelatin plate, five days at 22°. × 60. A colony in the early stage. The gray ring represents a zone where it is sinking in. VII. Agar plate, five days at 22°. Natural size. VIII. Agar plate, five days at 22°. X 60. Upper colony, superficial; lower colonies, deep. The superficial colonies usually become opaque toward the center. IX. Potato culture, eight days old. X. Microscopic preparation. Pure culture from agar. × 1000. Stained with fuchsin, differentiated with acetic acid. XI. Microscopic preparation. Pure culture in bouillon. X 1000. Unstained, partly schematic. |