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PLATE 43.

Bacillus mesentericus. Lehm. and Neum. (B. mesen

tericus fuscus Flügge.)

Natural size.

Natural size.

I. Gelatin stab culture, two days at 22°. II. Agar streak culture, three days at 22°. III. Potato culture, one day at 22°. IV. Potato culture, five days at 22°. V. Agar plate, two days at 22°. VI. Agar stab culture, four days at 22°. Surface growth.

Natural size.

VII. Agar plate, two days at 22°. X 60. Above, superficial; below, deep colonies.

VIII. Gelatin plate, thirty-six hours at 22°. × 60. Deep colonies.

IX. Gelatin plate, thirty-six hours at 22°. X 60. Superficial colony.

X. Gelatin plate, two days at 22°. Natural size.

XI. Gelatin plate, one day at 22°. × 60. To the right, deep; to the left, superficial colonies.

XII. Microscopic preparation. × 800. culture on agar two days old.

Some bacilli contain spores.

From a pure

Stained with fuchsin.

XIII. Bacilli with numerous flagella,

by Löffler's method.

X 1000. Stained

XIII.

Bacillus tetani.

PLATE 44.

Nicolaier. (Tetanus Bacillus.)

I. Sugar-agar stab culture, three days at 37°. II. Sugar-gelatin stab culture, six days at 22°. III. Sugar-gelatin plate, four days at 22°. without air.

Grown

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IV. Sugar-gelatin plate, four days at 22°. X 60. Natural size. Superficial and deep colonies. Grown without air. V. Sugar-agar plate, four days at 37°. Natural size. Grown without air.

VI. Sugar-agar plate, four days at 37°. X 60. Superficial and deep colonies. Grown without air.

VII. Microscopic preparation. Pure culture on sugaragar, three days at 37°. X 1000. Bacilli with spores. Double staining according to Ziehl.

VIII. Microscopic preparation. Pure culture on sugaragar, two days at 37°. X 1000. Some bacilli contain spores. Stained with fuchsin.

IX. Microscopic preparation. Pure culture on sugaragar, twenty-four hours at 37°. X 1000. Extremely long threads with faintly stained intervals.

X. Microscopic preparation. Pure culture on sugaragar, six days at 37°. X 1000. Stained with fuchsin. Long threads and chains of spores with faintly stained intervals. Flagella staining.

XI. Microscopic preparation.

Highly magnified.

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