PLATE 43. Bacillus mesentericus. Lehm. and Neum. (B. mesen tericus fuscus Flügge.) Natural size. Natural size. I. Gelatin stab culture, two days at 22°. II. Agar streak culture, three days at 22°. III. Potato culture, one day at 22°. IV. Potato culture, five days at 22°. V. Agar plate, two days at 22°. VI. Agar stab culture, four days at 22°. Surface growth. Natural size. VII. Agar plate, two days at 22°. X 60. Above, superficial; below, deep colonies. VIII. Gelatin plate, thirty-six hours at 22°. × 60. Deep colonies. IX. Gelatin plate, thirty-six hours at 22°. X 60. Superficial colony. X. Gelatin plate, two days at 22°. Natural size. XI. Gelatin plate, one day at 22°. × 60. To the right, deep; to the left, superficial colonies. XII. Microscopic preparation. × 800. culture on agar two days old. Some bacilli contain spores. From a pure Stained with fuchsin. XIII. Bacilli with numerous flagella, by Löffler's method. X 1000. Stained XIII. Bacillus tetani. PLATE 44. Nicolaier. (Tetanus Bacillus.) I. Sugar-agar stab culture, three days at 37°. II. Sugar-gelatin stab culture, six days at 22°. III. Sugar-gelatin plate, four days at 22°. without air. Grown IV. Sugar-gelatin plate, four days at 22°. X 60. Natural size. Superficial and deep colonies. Grown without air. V. Sugar-agar plate, four days at 37°. Natural size. Grown without air. VI. Sugar-agar plate, four days at 37°. X 60. Superficial and deep colonies. Grown without air. VII. Microscopic preparation. Pure culture on sugaragar, three days at 37°. X 1000. Bacilli with spores. Double staining according to Ziehl. VIII. Microscopic preparation. Pure culture on sugaragar, two days at 37°. X 1000. Some bacilli contain spores. Stained with fuchsin. IX. Microscopic preparation. Pure culture on sugaragar, twenty-four hours at 37°. X 1000. Extremely long threads with faintly stained intervals. X. Microscopic preparation. Pure culture on sugaragar, six days at 37°. X 1000. Stained with fuchsin. Long threads and chains of spores with faintly stained intervals. Flagella staining. XI. Microscopic preparation. Highly magnified. |