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Bacillus anthracis. F. Cohn and R. Koch.

(Splenic Fever.) I. Agar plate, four days at 22° X 60. To the left, a superficial colony; to the right, a colony directly below the surface; below, a deep colony.

II. Agar plate, four days at 22o. Natural size.

III. Agar plate, thirty-six hours at 37°. X 150. Surface growth. Peripheral part of a streak culture.

IV. Agar plate, thirty-six hours at 37° X 150. Deep colony.

V. Gelatin plate, three days at 22o. Natural size.

VI. Gelatin plate, three days at 22o. X 60. Superficial colony at the time of sinking in.

VII. Potato culture, six days at 22o. Natural size.

PLATE 36.

Bacillus anthracis. F. Cohn and R. Koch.

(Splenic Fever.)

I. Smear preparation from the blood of the spleen of a mouse. X 1000.

II. Contact preparation of an agar plate culture, one day at 22o. x 1000.

III. Unstained preparation in hanging drop from a bouillon culture, thirty-six hours at 37°. X 1000. Spores begin already to escape.

IV. Anthrax threads from agar, thirty-six hours at 37°. X 1000. Stained with Ziehl's solution; spores red, bacilli

blue.

V. Involution forms. Five-weeks'-old agar stab culture, stained with fuchsin. X 1000.

VI. Unstained preparation in hanging drop from a bouillon culture, eight hours at 370. X 1000. Beginning of spore-formation.

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