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Bacillus subtilis (Ehrenberg). F. Cohn.

(Hay Bacillus.)
I. Gelatin stab culture, thirty-six hours at 22°.
II. Gelatin stab culture, eight days at 22o.
III. Agar streak culture, two days at 37o.
IV. Agar stab culture, two days at 37°. Stab canal.

V. Agar stab culture, two days at 37o. Surface growth.

VI. Agar plate, twelve hours at 37°. X 60. Superficial colony.

VII. Agar plate, twelve hours at 37°. X 60. Deep colony.

VIII. Agar plate, twelve hours at 37°. Natural size.

Bacillus subtilis (Ehrenberg). F. Cohn.

(Hay Bacillus.)
I. Potato culture, seven days at 22o.

II. Gelatin plate, two days at 22°. X 60. Above and to the left, a deep colony; below this, one lying directly at the surface; to the right, a superficial colony.

III. Gelatin plate, two days at 22o. Natural size.
IV. Gelatin plate, two days at 22o. X 10.

V. Microscopic preparation. X 1000. From an agar culture three hours old at 37°, stained with fuchsin.

VI. Microscopic preparation. Bacilli with flagella, after Fischer. Very highly magnified.

VII. Microscopic preparation. X 1000. From an agar culture, ten days at 22o. Contains spores. Unstained.

VIII. Microscopic preparation. X 700. From an agar culture, ten days at 22°. Double stain with carbol-fuchsin and methylene-blue.

IX. Bacilli with numerous flagella. X 1000. Stained according to Löffler.

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