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PLATE 23.

Bacterium violaceum (J. Schröter). Lehm. and Neum.

I. Gelatin stab culture, ten days at ordinary temperature.

II. Agar streak culture, six days at ordinary temperature. The white borders after a longer time become likewise violet.

III. Agar stab culture, seven days at ordinary temperature. Stab canal.

IV. Agar stab culture, seven days at ordinary temperature. Surface growth.

V. Agar plate culture, four days at ordinary temperature. X 60. Superficial and deep colonies. Within the former is to be seen the original deeply located colony.

VI. Agar plate culture, eight days at ordinary temperature. Natural size. The colonies often also become dark violet.

VII. Gelatin plate culture. Natural size. Six days at ordinary temperature. The blue zones are not always so intensely colored.

VIII. Gelatin plate culture, six days at ordinary temperature. X 60. The smaller colony lies near the surface, the larger is on the surface.

IX. Microscopic preparation. X 700. From a fivedays'-old agar culture.

X. Potato culture, six days at ordinary temperature.
XI. Bacteria with flagella. × 1000. Stained accord-

ing to Löffler.

XII. Bacteria with flagella. × 1000. from Sweden.

From a culture

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PLATE 24.

Bacterium pyocyaneum (Flügge). Lehm. and Neum.

(Green Pus.)

I. Gelatin stab culture, three days at 22°.
II. Agar streak culture, two days at 37°.

III. Gelatin plate, two days at 22°. X 60. Colonies located deeply and just below the surface, in young and older stages.

IV. Gelatin plate, five days at 22°. X 60. Part of a superficial colony.

Natural size.

V. Gelatin plate, two days at 22°. Natural size.
VI. Agar plate, two days at 37°.
VII. Agar plate, two days at 37°.
superficial; below, deep colonies.

X 60. Above,

VIII. Potato culture, three days at 37°. Natural size. IX. Microscopic preparation. Pure culture from agar plate. 800.

X. Bacteria with one, more rarely two polar flagella. X 1000. Stained according to Löffler.

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