PLATE 35.

Bacillus anthracis. F. Cohn and R. Koch.

(Splenic Fever.)

I. Agar plate, four days at 22°. X 60. To the left, a superficial colony; to the right, a colony directly below the surface; below, a deep colony.

II. Agar plate, four days at 22°. Natural size.

III. Agar plate, thirty-six hours at 37°. X 150. Surface growth. Peripheral part of a streak culture.

IV. Agar plate, thirty-six hours at 37°. X 150. Deep colony.

V. Gelatin plate, three days at 22°. Natural size. VI. Gelatin plate, three days at 22°. X 60. Superficial colony at the time of sinking in.

VII. Potato culture, six days at 22°. Natural size.

mouse.

PLATE 36.

Bacillus anthracis.

F. Cohn and R. Koch.

(Splenic Fever.)

I. Smear preparation from the blood of the spleen of a
X 1000.

II. Contact preparation of an agar plate culture, one day at 22°. X 1000.

III. Unstained preparation in hanging drop from a bouillon culture, thirty-six hours at 37°. 1000. Spores begin already to escape.

IV. Anthrax threads from agar, thirty-six hours at 37°. X 1000. Stained with Ziehl's solution; spores red, bacilli blue.

V. Involution forms. Five-weeks'-old agar stab culture, stained with fuchsin. x 1000.

VI. Unstained preparation in hanging drop from a bouillon culture, eight hours at 37°. X 1000. Beginning of spore-formation.