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Mycobacterium lacticola

perrugosum. L. and N.

I. Streak culture (glycerin-agar), two months old; three days at 37°, then at 22°. Much elevated and wrinkled. II. Plate culture (glycerin-agar), six days at 37°. X 60. Superficial colony.

III. Plate culture (glycerin-agar), forty-eight hours at 37°. X 60. Superficial colony.

IV. Plate culture (glycerin-agar), three days at 37°. Natural size. Superficial colonies. Later the same were larger, more wrinkled, and reddish.

V. Potato culture, six days at 22°. Later the same became still more wrinkled.

VI. Microscopic preparation. Glycerin-agar: (a) Three days at 37°. X 1000. Stained with fuchsin. (b) Two months at 22°. X 1000. Stained with fuchsin.

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VII. Microscopic preparation. Smear from the peritoneal fluid of a guinea-pig, inoculated with butter. × 1000. Stained with tubercle bacillus stain. (From a preparation of Dr. Dieudonné.)

Mycobacterium phlei. Lehm. and Neum.

VIII. Streak culture (glycerin-agar), eight days at 22°. The culture at first is pale orange; later, it becomes darker and wrinkled.

IX. Plate culture (glycerin-agar), three days at 22°. X 60. Superficial colony.

X. Plate culture (glycerin-agar), eight days at 22°. × 60. Superficial colony.

XI. Plate culture (glycerin-agar), eight days at 22°. Natural size. Superficial colonies.

XII. Microscopic preparation. Glycerin-agar: (a) Three days at 37°. X 1000. Stained with fuchsin. (b) Two months at 22°. X 1000. Stained with fuchsin.

PLATE 64.

Mycobacterium lacticola a planum. L. and N.

α

I. Streak culture (ordinary agar), two months at 22°.
II. Streak culture (glycerin-agar), six days at 37°.

III. Streak culture (glycerin-agar), three months at 22°. The culture at first is whitish; later, it becomes very intensely orange-red.

IV. Streak culture (gelatin), six days at 22°.

V. Potato culture, six days at 22°. Its appearance varies widely. Sometimes it is lighter, sometimes darker, sometimes moister, sometimes drier, sometimes smooth, sometimes wrinkled.

VI. Plate culture (gelatin), six days at 22°. X 60. (a) Superficial colony, resembling the colon; (b) deep colony. VII. Plate culture (gelatin), six days at 22°. Natural size.

VIII. Plate culture (glycerin-agar), three days at 37°. X 60. Superficial colony.

IX. Plate culture (glycerin-agar), three days at 37°. Natural size.

X. Microscopic preparation. Glycerin-agar, three days. 1000. Stained with fuchsin. The size of the rods scarcely varies in very old cultures. There as here are found small and large, slender and thick rods.

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