National Institutes of Health Bulletin, Issue 180U.S. Government Printing Office, 1950 |
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alcohol ameboid trophozoites appear asexual Azure band form blood smears cells containing chro chromatin mass clean clumped compact cryptozoites cynomolgi cytoplasm dark DIAGNOSIS OF MALARIA Diaphane differentiate distilled divisions of chromatin Double infection dyes elongated enlarged cell eosinate erythrocytic examination falciparum malaria frequently gametocyte Garnham Giemsa stain growing trophozoites Health Rep infected cells irregular laboratory large numbers malaria parasites mass of chromatin matin Mature microgametocyte Mature schizont Mature trophozoite merozoites methylene blue microgametocyte microscopic mixed infections mosquito nucleus older trophozoites ovale peripheral blood pigment pigment granules Plasmodium cynomolgi Plasmodium malariae Plasmodium vivax pre-erythrocytic presegmenting schizonts red blood cells red cells resemble ring form trophozoite ring stage rounded schizogony Schüffner's stippling segmentation shape Shortt showing slide Small trophozoites sporozoites stain solution technician tertian Thick Blood Films thick film method thick film technique thin film usually vacuoles white cells xylol young ring forms young trophozoites
Popular passages
Page 6 - Double infection of single cell with young trophozoitcs, one a "marginal form," the other "signet ring" form. 3. 4. Young trophozoites showing double chromatin dots. 5, 6, 7. Developing trophozoite forms. 8. Three medium trophozoites in one cell. 9. Trophozoite showing pigment, in a cell containing Maurer's spots.
Page 37 - SOURCES OF CONFUSION OR ERROR The inexperienced microscopist may be confused in the examination of thick films by bacteria or dirt from the skin; by dust particles on the slide; by vegetable spores, yeast cells or fungi from the air; and by bacteria, molds, protozoa, or other contaminants from the distilled water used in staining. Probably the chief source of dirt on the thick film is the improperly cleaned skin.
Page 4 - Early band form trophozoite — elongated chromatin, some pigment apparent. 7. 8, 9, 10, 11, 12. Some forms which the developing trophozoite of quartan may take. 13, 14. Mature trophozoites — one a band form. 15, 16, 17, 18, 19. Phases in the development of the schizont ("presegmenting schizonts").
Page 27 - KH2PO4M/lo = 9.07 grams per liter. The two stock solutions are kept in separate glass-stoppered pyrex bottles from which are removed the following quantities to make the indicated amount of the buffered water: The pH of the buffered water is then tested and adjustments made if necessary. Brom-thymol blue indicator solution and a set of brom-thymol blue color standards are used for testing. Buffered water should be prepared each week. If this is inconvenient, the neutral water may be put into a large...
Page 2 - Schiiffner's stippling. i 6. 7. Very tenuous medium trophozoite forms. 8. Three ameboid trophozoites with fused cytoplasm. 9, 11, 12, 13. Older ameboid trophozoites in process of development. 10. Two ameboid trophozoites in one cell. 14. Mature trophozoite. 15. Mature trophozoite with chromatin apparently in process of division. 16. 17, 18, 19. Schizonts showing progressive steps in division ("presegmenting schizonts").
Page 23 - ... dehemoglobinization of the red cells which is part of the staining process. Hence all alcohol must be wiped away or allowed to evaporate before blood is taken for the smear. Some workers prefer to prick through the alcohol on the skin and then wipe away the alcohol with the first drop of blood.
Page 17 - However, in some heavy infections rare ones may occur usually along with large numbers of ring forms. The old trophozoites consist of a small bit of compact, light-staining cytoplasm, a granule of chromatin somewhat larger than in the ring and usually a dull blur or a small, dense, almost black block of pigment. The parasite at this stage is very small, sometimes hardly as large in circumference as the older ring forms. The presegmenting and segmented schizonts resemble those of the other species;...
Page 4 - P. malariae 1. Young ring form trophozoite of quartan malaria. 2, 3, 4. Young trophozoite forms of the parasite showing gradual increase of chromatin and cytoplasm. 5. Developing ring form trophozoite showing pigment granule.
Page 10 - The thick film gives an idea of the degree of infection and can be a great help, because of the increased density of parasites, in identifying the type of malaria in cases where only one or two young forms can be found in the thin film. It gives a fairly good idea of the number of leukocytes and shows pigmented white cells more readily than the thin film. It also makes...
Page 34 - ... except in heavy infections and is consequently usually accompanied by large numbers of ring forms. When it is found, it is usually very small, compact, often nonvacuolated. The chromatin dot is larger than that in the young ring; the cytoplasm seems lighter than that of the similar stage of P.