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Bacillus vulgatus. Migula. (B. mesentericus vulgatus

Flügge. Potato Bacillus.)
I. Gelatin stab culture, ten days at 22o.
II. Agar streak culture, ten days at 22o.
III. Agar stab culture, six days at 22°. Surface growth.
IV. Agar plate, six days at 22o. Natural size.

V. Agar plate, six days at 22°. X 60. Deep colonies.

VI. Agar plate, six days at 22° X 60. Superficial colony.

VII. Gelatin plate, eight days at 22o. Natural size. VIII. Gelatin plate, eight days at 22°. X 60. Part of a superficial colony. IX. Gelatin plate, eight days at 22o. X 150.

Part of a superficial colony.

X. Potato culture, five days at 22o. Natural size.

XI. Microscopic preparation. Pure culture from agar, one day old. X 800. Stained with fuchsin.

XII. Bacilli with numerous flagella. X 1000. Stained according to Löffler.

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Bacillus mesentericus. Lehm. and Neum. (B. mesen

tericus fuscus Flügge.)

I. Gelatin stab culture, two days at 22o.
II. Agar streak culture, three days at 22o.
III. Potato culture, one day at 22o. Natural size.
IV. Potato culture, five days at 22o. Natural sizė.

V. Agar plate, two days at 22° Natural size.

VI. Agar stab culture, four days at 22°. Surface growth.

VII. Agar plate, two days at 22o. X 60. Above, superficial; below, deep colonies.

VIII. Gelatin plate, thirty-six hours at 22o. X 60. Deep colonies.

IX. Gelatin plate, thirty-six hours at 22o. x 60. Superficial colony.

X. Gelatin plate, two days at 22° Natural size.

XI. Gelatin plate, one day at 22° X 60. To the right, deep; to the left, superficial colonies.

XII. Microscopic preparation. X 800. From a pure culture on agar two days old.

Stained with fuchsin. Some bacilli contain spores.

XIII. Bacilli with numerous flagella. X 1000. Stained by Löffler's method.

Bacillus tetani. Nicolaier. (Tetanus Bacillus.)

I. Sugar-agar stab culture, three days at 37°.
II. Sugar-gelatin stab culture, six days at 22°.

III. Sugar-gelatin plate, four days at 22o. Grown without air.

IV. Sugar-gelatin plate, four days at 22o. X 60. Natural size. Superficial and deep colonies. Grown without air.

V. Sugar-agar plate, four days at 37o. Natural size. Grown without air.

VI. Sugar-agar plate, four days at 37° X 60. Superficial and deep colonies. Grown without air.

VII. Microscopic preparation. Pure culture on sugaragar, three days at 370. X 1000. Bacilli with spores. Double staining according to Ziehl.

VIII. Microscopic preparation. Pure culture on sugaragar, two days at 37° X 1000. Some bacilli contain spores. Stained with fuchsin.

IX. Microscopic preparation. Pure culture on sugaragar, twenty-four hours at 37° X 1000. Extremely long threads with faintly stained intervals.

X. Microscopic preparation. Pure culture on sugaragar, six days at 37°. X 1000. Stained with fuchsin. Long threads and chains of spores with faintly stained intervals.

XI. Microscopic preparation. Flagella staining. Highly magnified.

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