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Bacterium pestis. Lehm. and Neum.

I. Streak culture (ascites-glycerin-agar), three days at 37°

II. Streak culture (agar), forty-eight hours at 37°. (After a culture of Dr. Dieudonné, preserved with formalin.) The streak was made with the juice direct from a bubo. The transparent, dewdrop-like growth is characteristic.

III. Stab culture (gelatin), six days at 22o. The growth consists of minute, waxy, markedly elevated colonies, which become confluent; also the same upon the gelatin plate (v, b).

IV. Plate culture (gelatin), six days at 22°. X 60. (a) Deep.colony; (b) superficial colony.

V. Plate culture: (a) Glycerin-agar, three days at 37°. Natural size. Superficial colonies. (6) Gelatin, six days at 22o. Natural" size. Superficial colonies. Compare what is said under Fig. III.

VI. Plate culture (agar), forty-eight hours at 37° X 60. Superficial colonies. They correspond to the dewdrop-like colony in the agar streak culture (11). (a) Younger, (b) older colonies. VII. Plate cultures, forty-eight hours at 37°.

X 60. (a) Ordinary agar (6) Glycerin-agar Superficial colonies. (c) Ascites-glycerin-agar

(d) Ascites-glycerin-agar, deep colony. The crumbly character of cultures after being cultivated in contrast to very fresh cultures is to be noted (compare vi).

VIII. Microscopic preparation, three days at 37° on glycerin-agar. X 1000. Stained with fuchsin. Involution forms.

IX. Microscopic preparation: Smear from the juice of a bubo. X 1000. Stained with methylene-blue. (From a preparation of Dr. Dieudonné.)

X. Microscopic preparation : (a) Ordinary agar, twenty-four hours at 37°. X 1000. Stained with fuchsin. (6) Ordinary bouillon, twenty-four hours at 37°. X 1000.

Bacterium acidi lactici. Hüppe. (Lactic Acid Bacillus.)

I. Gelatin stab culture, five days at 22o. The stab canal in nature is somewhat whiter.

II. Agar streak culture, five days at 22o.
III. Agar stab culture, three days at 22°. Stab canal.

IV. Agar stab culture, three days at 22°. Surface growth.

V. Agar plate, three days at 22o. Natural size.

VI. Agar plate, three days at 22°. X 50. Upper colony superficial, lower colonies deep. Compare also Plate 18, VII.

VII. Gelatin plate, two days at 22o. Natural size. VIII. Gelatin plate, two days at 22o. X 50. Upper colony superficial, lower colonies deep. The superficial colonies may vary very much. Compare also Plate 16, VIII, IX; Plate 17, I, II; Plate 19, IV, VII.

IX. Microscopic preparation. Pure culture from an agar colony. X 800.

X. Potato culture, six days at 22o. The air-bubbles on the surface often cover it completely.

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