The Elements of Bacteriological Technique: A Laboratory Guide for the Medical, Dental, and Technical StudentW.B. Saunders, 1902 - 361 pages |
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Page 141
... and store in 1 - litre flask . 3. Inoculate the bouillon from a pure cultivation of the B. lactis aerogenes , and incubate at 37 ° C. for forty - eight hours . 4. Heat in the steamer at 100 ° C. for 141 STOCK CULTURE MEDIA.
... and store in 1 - litre flask . 3. Inoculate the bouillon from a pure cultivation of the B. lactis aerogenes , and incubate at 37 ° C. for forty - eight hours . 4. Heat in the steamer at 100 ° C. for 141 STOCK CULTURE MEDIA.
Page 142
... incubate at 37 ° C. for forty - eight hours . 7. Heat in the steamer at 100 ° C. for twenty minutes . Now fill two fermentation tubes with the bouillon , tint with litmus solution , and sterilise ; inoculate with B. lactis aerogenes ...
... incubate at 37 ° C. for forty - eight hours . 7. Heat in the steamer at 100 ° C. for twenty minutes . Now fill two fermentation tubes with the bouillon , tint with litmus solution , and sterilise ; inoculate with B. lactis aerogenes ...
Page 144
... Incubate at 37 ° C. for forty - eight hours to eliminate contaminated tubes . Store the remainder for future use . Carbolised Bouillon.- 6 ) . 1. Prepare nutrient bouillon ( vide page 141 , sections I to 2. Weigh out carbolic acid , 1 ...
... Incubate at 37 ° C. for forty - eight hours to eliminate contaminated tubes . Store the remainder for future use . Carbolised Bouillon.- 6 ) . 1. Prepare nutrient bouillon ( vide page 141 , sections I to 2. Weigh out carbolic acid , 1 ...
Page 156
... Incubate at 37 ° C. for forty - eight hours to eliminate any contaminated tubes . Store the remainder for future use . Ascitic Bouillon ( Serum Bouillon ) .- 1. Collect ascitic fluid ( pleuritic fluid , hydrocele fluid , etc. , by ...
... Incubate at 37 ° C. for forty - eight hours to eliminate any contaminated tubes . Store the remainder for future use . Ascitic Bouillon ( Serum Bouillon ) .- 1. Collect ascitic fluid ( pleuritic fluid , hydrocele fluid , etc. , by ...
Page 157
... incubate at 37 ° C. for forty - eight hours , to eliminate any contaminated tubes . Store the remainder for future use . Serum Agar ( Heiman ) .— 1. Prepare nutrient agar ( vide page 149 ) , to follow- ing formula : agar , 2 per cent ...
... incubate at 37 ° C. for forty - eight hours , to eliminate any contaminated tubes . Store the remainder for future use . Serum Agar ( Heiman ) .— 1. Prepare nutrient agar ( vide page 149 ) , to follow- ing formula : agar , 2 per cent ...
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Common terms and phrases
absolute alcohol acid anaerobically aniline animal Apparatus Required aqueous solution Bacillus bacteria beaker blood-serum boiling bottle bouillon Bunsen Bunsen burner c.c. capacity cell cent Clinical Cloth colonies colour containing cotton-wool plug cover-slip films cubic centimetre culture tube cylinder dilute Diseases dissolve distilled water Edition emulsify emulsion filter candle Filter through papier flame flask fluid forceps fuchsin funnel gelatine gentian violet glass tubing glucose glucose formate Gram's method grammes growth guinea-pig Half Morocco Handsome octavo hanging-drop heat incubate incubate at 37 Inoculate inoculum meat extract Medicine medium micrometer NaOH nutrient agar nutrient bouillon nutrient gelatine octavo organisms papier Chardin peptone pipette Professor quantity reaction Remove sample sections serum Sheep or Half slide soda solution spores steamer at 100 sterile sterile capsules sterile pipettes sterilise stopper surface Surgery temperature test-tube tion tissue tube cultivations tubes of nutrient twenty minutes vide water-bath Weigh xylol