The Elements of Bacteriological Technique: A Laboratory Guide for the Medical, Dental, and Technical StudentW.B. Saunders, 1902 - 361 pages |
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Page 128
... sample of meat extract , it is essential that- 1. The meat extract be previously exposed to a tem- perature of 100 ° C. for forty - five minutes . 2. The estimation be performed at the boiling - point . 3. Phenolphthalein be used as the ...
... sample of meat extract , it is essential that- 1. The meat extract be previously exposed to a tem- perature of 100 ° C. for forty - five minutes . 2. The estimation be performed at the boiling - point . 3. Phenolphthalein be used as the ...
Page 130
... Sample of filtered meat extract or nutrient gelatine to which phenolphthalein has been added . The medium is acid , as evidenced by the unaltered colour of the sample . b , The same neutralised by the addition of n IO NaOH . The ...
... Sample of filtered meat extract or nutrient gelatine to which phenolphthalein has been added . The medium is acid , as evidenced by the unaltered colour of the sample . b , The same neutralised by the addition of n IO NaOH . The ...
Page 131
... sample of meat extract which reacts acid to phenol- phthalein , and would require the addition of 10 c.c. of normal NaOH per litre , to neutralise it . NOTE . Such a solution would probably react alka- line to litmus . Conversely , if ...
... sample of meat extract which reacts acid to phenol- phthalein , and would require the addition of 10 c.c. of normal NaOH per litre , to neutralise it . NOTE . Such a solution would probably react alka- line to litmus . Conversely , if ...
Page 161
... sample of milk and proceed with another litre of milk from a different source . Carefully eliminate milk to which antiseptics have been added as preservatives . 3. Allow the milk to cool , when the fat or cream will rise to the surface ...
... sample of milk and proceed with another litre of milk from a different source . Carefully eliminate milk to which antiseptics have been added as preservatives . 3. Allow the milk to cool , when the fat or cream will rise to the surface ...
Page 193
... 5. Attach a piece of rubber tubing to the exit tube b and collect samples of the issuing gas ( over water ) and test from time to time . 6. When the air is completely displaced by hydrogen , 13 ANAEROBIC CULTIVATIONS . 193.
... 5. Attach a piece of rubber tubing to the exit tube b and collect samples of the issuing gas ( over water ) and test from time to time . 6. When the air is completely displaced by hydrogen , 13 ANAEROBIC CULTIVATIONS . 193.
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absolute alcohol acid anaerobically aniline animal Apparatus Required aqueous solution Bacillus bacteria beaker blood-serum boiling bottle bouillon Bunsen Bunsen burner c.c. capacity cell cent Clinical Cloth colonies colour containing cotton-wool plug cover-slip films cubic centimetre culture tube cylinder dilute Diseases dissolve distilled water Edition emulsify emulsion filter candle Filter through papier flame flask fluid forceps fuchsin funnel gelatine gentian violet glass tubing glucose glucose formate Gram's method grammes growth guinea-pig Half Morocco Handsome octavo hanging-drop heat incubate incubate at 37 Inoculate inoculum meat extract Medicine medium micrometer NaOH nutrient agar nutrient bouillon nutrient gelatine octavo organisms papier Chardin peptone pipette Professor quantity reaction Remove sample sections serum Sheep or Half slide soda solution spores steamer at 100 sterile sterile capsules sterile pipettes sterilise stopper surface Surgery temperature test-tube tion tissue tube cultivations tubes of nutrient twenty minutes vide water-bath Weigh xylol