The Elements of Bacteriological Technique: A Laboratory Guide for the Medical, Dental, and Technical StudentW.B. Saunders, 1902 - 361 pages |
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Page 35
... spores , etc. , in about thirty minutes , whilst a few minutes ' exposure to a temperature of 170 ° to 180 ° C. will effect the same result . This method is only applicable to glass and metallic substances , and the small bulk of cot ...
... spores , etc. , in about thirty minutes , whilst a few minutes ' exposure to a temperature of 170 ° to 180 ° C. will effect the same result . This method is only applicable to glass and metallic substances , and the small bulk of cot ...
Page 37
... spores . Its use is limited to the sterilisa- tion of such albuminous " fluid " media as would co- agulate at a higher temperature . METHOD.- 1. Fit up a water - bath , heated by a Bunsen flame which is controlled by a thermo ...
... spores . Its use is limited to the sterilisa- tion of such albuminous " fluid " media as would co- agulate at a higher temperature . METHOD.- 1. Fit up a water - bath , heated by a Bunsen flame which is controlled by a thermo ...
Page 38
... spores will have germinated and assumed the vegetative form . 5. Destroy these new vegetative forms by a similar exposure to 56 ° C. on the second day , whilst others , of slower germination , may be caught on the third day , and so on ...
... spores will have germinated and assumed the vegetative form . 5. Destroy these new vegetative forms by a similar exposure to 56 ° C. on the second day , whilst others , of slower germination , may be caught on the third day , and so on ...
Page 41
... spores present will germinate , and the young organism will be de- stroyed by repeating the process twenty - four hours later ; a third sterilisation after a similar interval makes assurance doubly sure . The method of sterilising by ...
... spores present will germinate , and the young organism will be de- stroyed by repeating the process twenty - four hours later ; a third sterilisation after a similar interval makes assurance doubly sure . The method of sterilising by ...
Page 58
... temperature of hang- ing - drop preparations , etc. , placed upon it , above that of the surrounding atmosphere , is extremely useful for observations on spore germination , hanging - drop cultivations 58 THE MICROSCOPE .
... temperature of hang- ing - drop preparations , etc. , placed upon it , above that of the surrounding atmosphere , is extremely useful for observations on spore germination , hanging - drop cultivations 58 THE MICROSCOPE .
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Common terms and phrases
absolute alcohol acid anaerobically aniline animal Apparatus Required aqueous solution Bacillus bacteria beaker blood-serum boiling bottle bouillon Bunsen Bunsen burner c.c. capacity cell cent Clinical Cloth colonies colour containing cotton-wool plug cover-slip films cubic centimetre culture tube cylinder dilute Diseases dissolve distilled water Edition emulsify emulsion filter candle Filter through papier flame flask fluid forceps fuchsin funnel gelatine gentian violet glass tubing glucose glucose formate Gram's method grammes growth guinea-pig Half Morocco Handsome octavo hanging-drop heat incubate incubate at 37 Inoculate inoculum meat extract Medicine medium micrometer NaOH nutrient agar nutrient bouillon nutrient gelatine octavo organisms papier Chardin peptone pipette Professor quantity reaction Remove sample sections serum Sheep or Half slide soda solution spores steamer at 100 sterile sterile capsules sterile pipettes sterilise stopper surface Surgery temperature test-tube tion tissue tube cultivations tubes of nutrient twenty minutes vide water-bath Weigh xylol