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Bacterium pestis. Lehm. and Neum.
I. Streak culture (ascites-glycerin-agar), three days at 37°
II. Streak culture (agar), forty-eight hours at 37° (After a culture of Dr. Dieudonné, preserved with formalin.) The streak was made with the juice direct from a bubo. The transparent, dewdrop-like growth is characteristic.
III. Stab culture (gelatin), six days at 22°. The growth consists of minute, waxy, markedly elevated colonies, which become confluent; also the same upon the gelatin plate (v, b).
IV. Plate culture (gelatin), six days at 22°. X 60. (a) Deep colony; (b) superficial colony.
V. Plate culture: (a) Glycerin-agar, three days at 37o. Natural size. Superficial colonies. (6) Gelatin, six days at 22o. Natural size. Superficial colonies. Compare what is said under Fig. III.
VI. Plate culture (agar), forty-eight hours at 37°.
(a) Ordinary agar
Superficial colonies. (c) Ascites-glycerin-agar
(d) Ascites-glycerin-agar, deep colony. The crumbly character of cultures after being cultivated in contrast to very fresh cultures is to be noted (compare VI).
VIII. Microscopic preparation, three days at 37° on glycerin-agar. X 1000. Stained with fuchsin. Involution forms.
IX. Microscopic preparation: Smear from the juice of a bubo. X 1000. Stained with methylene-blue. (From a preparation of Dr. Dieudonné.)
X. Microscopic preparation : (a) Ordinary agar, , twenty-four hours at 370. X 1000. Stained with fuchsin. (6) Ordinary bouillon, twenty-four hours at 37o. X 1000. Stained with fuchsin,