Vibrio choleræ. (Koch.) Buchner. (Comma Bacillus.)

I. Gelatin plate, thirty-six hours at 22o. X 60. Deep and superficial colonies.

II. Gelatin plate, forty-eight hours at 22°. X 60. To the left superficial, to the right deep colonies.

III. Gelatin plate, three days at 22°. X 60. Superficial colonies with zone of liquefaction.

IV. Gelatin plate, three days at 22o. X 60. Deep colonies.

V. Gelatin plate, four days at 22°. X 60. Superficial colony with zone of liquefaction. VI. Gelatin plate, four days at 22°.

X 60. Deep colony.

VII. Gelatin plate, five days at 22o. x 60. Deep colony from a culture of cholera from Hanover. VIII. Gelatin plate, five days at 22° X 60. Superfi

. cial colony. Complete liquefaction has already taken place. IX. Gelatin plate, eight days at 22° X 60. Superfi

. cial colony with zone of liquefaction.

PLATE 50.

Vibrio choleræ. (Koch.) Buchner. (Comma Bacillus.)

I. Gelatin plate, five days at 22°. x 60. Abnormal form of superficial colony. II. Gelatin plate, five days at 22° X 90. Abnormal

. form of superficial colony. III. Gelatin plate, five days at 22° X 60. Deeply

. sunken superficial colony with a strongly reflecting zone of liquefaction. IV. Gelatin plate, six days at 22°. X 60. Abnormal

x superficial colony, with dense nucleus, evenly sunken in and with a zone of liquefaction.

V. Gelatin plate, six days at 22°. X 60. Abnormal deep colony, without halo, dark, with radiating striations, from the same plate as iv.

VI. Potato culture, two days at 22o. Natural size. . Soaked in soda solution before inoculation. VII. Potato culture, five days at 22o. Inoculated upon

. ordinary potato.